in Hawaii (Clausen, 1978) may have been expected to have some impact on B. cucurbitae as well as Bactrocera dorsalis. . We used culture‐based approaches to isolate and characterize gut‐associated bacterial species from the B. cucurbitae GSS during mass rearing and applied one of the isolated bacteria, Enterobacter spec., as a probiotic mixed into the larval diet to examine its effect on B. cucurbitae. In this study, we constructed life tables for Bactrocera cucurbitae on cucumber (Cucumis sativus L.) in the laboratory and under simulated field conditions. USA: Reference(s) Other studies have found that probiotics in the fruit fly larval diet can enhance host fitness. Potential diet regimens for laboratory rearing of the harlequin ladybird. Life table parameters of the tomato leaf miner Tuta absoluta (Lepidoptera: Gelechiidae) on different tomato cultivars. For example, the irradiation used for SIT disturbs microbe balance in the midgut for C. capitata and A. ludens, reducing the abundance of specific, functional gut bacteria (Niyazi et al., 2004; Ami et al., 2010; Estes et al., 2011; Gavriel et al., 2011; Jurkevitch, 2011; Zindel et al., 2011; Lauzon & Potter, 2012). Demographic Analysis of Sex Ratio on Population Growth of (Lepidoptera: Noctuidae) in 24 Sugar Beet Genotypes Population Growth of Dysaphis pyri (Hemiptera: Aphididae) on Different Pear Cultivars With Discussion on Curve Fitting in Life Table Studies. The effects of the probiotic larval diet on the size of irradiated adult B. cucurbitae males and females were measured using head width (HW), thorax length (TL), abdomen length (AL), and wing length (WL). (Coleoptera: Chrysomelidae), Fed on Alligator Weed Under Elevated CO Twenty‐nine and 25 colonies were sequenced from 5‐day‐old male and female guts, respectively. However, if cucumbers were kept in the field but were not covered, the r and R0 for the two experiments were 0.1043 and 0.0904 per day, and 27.7 and 10.1 offspring, respectively. This knowledge might help to further develop B. cucurbitae as another target insect species for SIT. Review of the role of gut microbiota in mass rearing of the olive fruit fly, actrocera oleae, and its parasitoids. . Life‐history and demographic traits of the marula fruit fly Ceratitis cosyra: potential consequences of host specialization. In the Solomon Islands it was subjected to an eradication campaign using a combination of bait spraying and male annihilation traps. A sterile inoculating loop was used to select a single colony from the LB solid plates to inoculate LB liquid medium in 1‐ml EP tubes under sterile conditions. Age-stage, two-sex life table: the basis of population ecology, biological control and pest management個体群生態学,生物的防除および害虫管理における基礎としての日齢−齢期両性生命表とその応用. Captures of Wild Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae (Diptera: Tephritidae) in Traps with Improved Multilure TMR Dispensers Weathered in California Roger I. Vargas 1 USDA-ARS, Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center, 64 Nowelo St., Hilo, HI 96720 ( roger.vargas@ars.usda.gov ; steven.souder@ars.usda.gov ), Bactrocera dorsalis One hundred pupae from each group were weighed 2 days before emergence. Evaluation of Sublethal Effects of Sulfoxaflor on the Green Peach Aphid (Hemiptera: Aphididae) Using Life Table Parameters. Koch (Acari: Tetranychidae) . Under Simulated Weather Conditions In Egypt Special Issue: Special Issue ‐ Improving Pest Control: Mass Rearing and Field Performance. Tephritid-microbial interactions to enhance fruit fly performance in sterile insect technique programs. Increased number of large non‐atretic follicles and co‐dominance effects account for high litter sizes in Bonga sheep. Survival and Development of Enter your email address below and we will send you your username, If the address matches an existing account you will receive an email with instructions to retrieve your username, Special Issue ‐ Improving Pest Control: Mass Rearing and Field Performance, I have read and accept the Wiley Online Library Terms and Conditions of Use, Gapped BLAST and PSI‐BLAST: a new generation of protein database search programs, Manipulation of the microbiota of mass‐reared Mediterranean fruit flies, Exploitation of the medfly gut microbiota for the enhancement of sterile insect technique: use of, Enterobacteria‐mediated nitrogen fixation in natural populations of the fruit fly, Structure and function of the bacterial community associated with the Mediterranean fruit fly, Gut bacterial communities in the Mediterranean fruit fly (, Bringing back the fruit into fruit fly‐bacteria interactions, Effect of bacteria on nutritional status and reproductive success of the Mediterranean fruit fly, Effects of probiotics on radiation‐induced intestinal injury in rats. These parasites lay their eggs in the eggs or maggots and emerge in the pupal stage. In this study, we constructed life tables for Bactrocera cucurbitae on cucumber (Cucumis sativus L.) in the laboratory and under simulated field conditions. To assess the variability of the life tables, we carried out two experiments under each treatment. Learn more. The original 3120 bp hopperBd-Kah element isolated from the Kahuku wild-type strain was highly degenerate and appeared to have a mutated transposase and terminal sequences, while a second … Effect of Arsenophonus Endosymbiont Elimination on Fitness of the Date Palm Hopper, Ommatissus lybicus (Hemiptera: Tropiduchidae). A B. cucurbitae genetic sexing strain (GSS) was established for SIT. Age-stage, two-sex life history of the golden twin spot moth, Trialeurodes vaporariorum Please check your email for instructions on resetting your password. Thirty-two species and varieties of natural enemies to fruit flies were introduced to Hawaii between 1947 and 1952 to control the fruit flies. Sterile insect technique (SIT) is a ‘hot’ research field for pest control, particularly in fruit flies. . PCR amplification was performed from the bacterial solution using the universal primers 968GC and L1401 described by Nubel et al. Age-Stage, Two-Sex Life Tables of the Lady Beetle (Coleoptera: Coccinellidae) Feeding on Different Aphid Species. Journal of the American Mosquito Control Association. Petri dishes (no lids) with 100 irradiated pupae (white or brown) were covered with cylinders. Demography and Mass-Rearing Harmonia dimidiata (Coleoptera: Coccinellidae) Using Aphis gossypii (Hemiptera: Aphididae) and Eggs of Bactrocera dorsalis (Diptera: Tephritidae). Heat treatment is one of the numerous effective quarantine treatments for Bactrocera flies (Heather and Hallman 2008). Effects of prolonged mass rearing on life history traits of There was no significant variation in flight ability among the different treatments (F2,6 = 0.894, P = 0.46). probiotics on emergence and flight ability in irradiated B. cucurbitae males was assessed, the PI and API groups exhibited no significant increase in the two quality control (QC) parameters relative to the NPI group (emergence: F2,6 = 2.595, P = 0.15; flight ability: F2,6 = 1.958, P = 0.22). Egyptian Journal of Biological Pest Control. The melon fly, Bactrocera cucurbitae, originally from the Indo-Malayan region, is an important Tephritid agricultural pest with a broad host of fruit and vegetables of tropical and sub-tropical regions.It was the first tephritid species introduced in Hawaii. Prior to dissection, the adult insects were surface‐sterilized with 70% ethanol for 2–5 min and then rinsed 3× in sterile distilled water. Fitness costs of sulfoxaflor resistance in the cotton aphid, Aphis gossypii Glover. Temperature‐dependent demography of griphila aeneociliella (Lepidoptera: Crambidae), a new insect pest of wheat in China. Degradation Capacity and Diapause Effects on Oviposition of Hermetia illucens (Diptera: Stratiomyidae). . The measurements were performed on a sample of 90 probiotic and 90 non‐probiotic males (including autoclaved probiotics) with a stereomicroscope fitted with Motic Images Plus software v.2 (Rodriguero et al., 2002). . Demography and Population Projection of Flea Beetle, The dead and live flies were counted after 48 h. Nine replicates were performed per group. (Noctuidae) Affected by Sugar Beet Cultivars Effects of three different cultivars of cruciferous plants on the age‐stage, two‐sex life table traits of Plutella xylostella (L.) (Lepidoptera: Plutellidae). ^ ARS | Publication request: Parasitization of Melon Fly (Diptera:tephritidae) by Fopius Arisanus and Psyttalia Fletcheri (Hymenoptera:braconidae) and the Effect of Fruit Substrates on Host Preference by Parasitoids , Ars.usda.gov. Learn about our remote access options, Laboratory of Theoretical and Applied Ecology, Department of Entomology, National Chung Hsing University, Taichung, Taiwan, Republic of China, Hsin Chi (corresponding author), P.O. Soybean aphid (Hemiptera: Aphididae) response to lambda‐cyhalothrin varies with its virulence status to aphid‐resistant soybean. Impact of constant versus fluctuating temperatures on the development and life history parameters of Tetranychus urticae (Acari: Tetranychidae). Bactrocera cucurbitae (Coquillett) is an important fruit and vegetable pest that can harm more than 120 types of agricultural products (Yuan et al., 2005; He et al., 2012). Effect of imidacloprid exposure on life history traits in the agricultural generalist predator Paederus beetle: Lack of fitness cost but strong hormetic effect and skewed sex ratio. Genetic and genomic analyses for predicted methane‐related traits in Japanese Black steers. Releases of Opius spp., Biosteres spp. The mean female and male pupal weight increased by 0.17 ± 0.04 and 0.08 ± 0.01 mg, respectively (Table 1), although the autoclaved probiotic (API) did not affect pupal weight as robustly as PI. The API group experienced only a slight weight increase in 0.04 ± 0.04 and 0.03 ± 0.01 mg (females and males, respectively) for pupal weight compared to the NPI group (Table 1). A few of the important pest species include: Asian fruit fly, Bactrocera invadens Carambola fly, Bactrocera carambolae Chinese citrus fruit fly, Bactrocera minax guava fruit fly,Bactrocera correcta Malaysian fruit fly,Bactrocera latifrons melon fruit fly, Bactrocera cucurbitae olive fruit fly, Bactrocera oleae oriental fruit fly, Bactrocera dorsalis peach frui… as determined by age-stage, two-sex life table Bensulfuron-Methyl Treatment of Soil Affects the Infestation of Whitefly, Aphid, and Tobacco Mosaic Virus on Nicotiana tabacum. . The possible implication of the API diet suggests an obvious advantage over a live diet because it is more convenient, secure, and efficient. Acta Phytopathologica et Entomologica Hungarica. We thank Mijuan Luo for providing guidance during the experiment. Temperature-dependent age-specific demography of grapevine moth ( : Gelechiidae): a case study on host plant resistance. Cue lure (4-(p-acetoxyphenyl)-2-butanone) attracts flies at very low concentrations and is believed to attract over a range of up to 1 km. Assessing Compatibility of the Predatory Mite However, survival rates varied significantly for the different treatments (F2,6 = 12.732, P<0.01); a greater increase was observed for treatment with API than for treatment with PI (18 vs. 12% increase). ) (Lepidoptera: Tortricidae): jackknife vs. bootstrap techniques ECOTOXICOLOGY Insecticidal Activity of Basil Oil, trans-Anethole, Estragole, and Linalool to Adult Fruit Flies of Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae CHIOU LING CHANG,1,2 IL KYU CHO,2,3 AND QING X. LI3,4 J. Econ. This finding suggests that the nutritional benefit of bacteria had a more significant influence than the insect–bacteria interaction, consistent with B. cucurbitae females placed in an environment with no food and water (Table 1). Low diversity bacterial community and the trapping activity of metabolites from cultivable bacteria species in the female reproductive system of the oriental fruit fly, Incidence of Enterobacteriaceae in the larvae of the polyphagous insect (, The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools, Comparison of the diversity of the bacterial communities in the intestinal tract of adult, Identification of cultivable bacteria in the intestinal tract of, Irradiation Influence on Melon Fly, Bactrocera cucurbitae (Coquillett), Arthropod symbioses: a neglected parameter in pest‐ and disease‐control programmes. Mass rearing and superior‐fit target species are crucial components of SIT. Transgenerational hormetic effects of sublethal dose of flupyradifurone on the green peach aphid, Myzus persicae (Sulzer) (Hemiptera: Aphididae). Screening of Potential Sources of Resistance to References Abstract Guide: Print: Email to a Friend « Previous Species Aspidiotus nerii Next Species Bactrocera dorsalis » Discuss this Record: Application of semiochemicals to control Bactrocera cucurbitae, the Melon fly. dominated both populations (37% in males and females), leading us to select the Enterobacter spec. The dominant bacteria in the fruit fly gut are from the Enterobacteriaceae family and include Enterobacter spec., Klebsiella spec., Providencia spec., Pectobacterium spec., and Citrobacter freundii (Braak) Werkman & Gillen (Lauzon, 2003; Niyazi et al., 2004; Behar et al., 2005, 2008a,b; Ami et al., 2010; Gavriel et al., 2011). . All of the sequences from 5‐day‐old male and female adults were identified as Enterobacter spec., Providencia spec., Cedecea spec., Lysinibacillus spec., and Klebsiella spec. isolated from 5‐day‐old male guts as the larval probiotic. The irradiation used in SIT not only damaged gut‐associated microbes (Demirer et al., 2006) but also affected the histology, ultrastructure, and physiological functions of fruit flies, indicating the need for additional research in this area (Lauzon & Potter, 2012). The ‘live probiotic’ (PI) diet and the ‘autoclaved probiotic’ (API) diet both significantly improved pupal weight, morphological indices, and adult survival rate compared with the control ‘non‐probiotic’ (NPI) diet group. Some reports have demonstrated that the irradiation used for SIT damages the midgut and tissues of target species, which can impair SIT success. was more significant than the insect–bacteria interaction in B. cucurbitae GSS (Behar et al., 2005), which is in accordance with our goal. To monitor the potential for development of resistance, information about the current levels of tolerance to spinosad in melon fly populations were established in this study. and an un-named Spalangia sp. The oesophageal diverticulum of Dirioxa pornia studied through micro-CT scan, dissection and SEM studies. The responses of cucumber plants subjected to different salinity or fertilizer concentrations and reproductive success of Tetranychus urticae mites on these plants. The BLAST analysis of the V6‐V8 16S rRNA gene segment sequences from the isolated colonies confirmed their identification as Enterobacter spec., Providencia spec., and Klebsiella spec., Cedecea spec., and Lysinibacillus spec. . The %emergence rate (E%) and fly‐out rate (F%) were measured: E% = 100% × [(100 − A‐B)/100], and F% = 100% × [(100 − A‐B‐C‐D)/100]. Lure and kill. Attractancy potential of culturable bacteria from the gut of peach fruit fly, Attraction of Mexican fruit flies (Diptera: Tephritidae) to bacteria: effects of culturing medium on odour volatiles, Morphometric traits and sexual selection in medfly (Diptera: Tephritidae) under field cage conditions, Development of probiotic diets for the olive fly: evaluation of their effects on fly longevity and fecundity. How Different Genetically Manipulated Brassica Genotypes Affect Life Table Parameters of Plutella xylostella (Lepidoptera: Plutellidae). Differential abundance and core members of the bacterial community associated with wild male Zeugodacus cucurbitae fruit flies (Insecta: Tephritidae) from three geographical regions of Southeast Asia. Effectiveness of mass trapping by bottle traps baited with salt sardines to control Bactrocera oleae (Gmelin) Virgilio Caleca , Matteo Maltese Scienze Agrarie, Alimentari e Forestali 1). Poppius (Hemiptera: Anthocoridae) on Reports evaluating B. cucurbitae in the context of SIT have largely focused on examining ecology, mating behavior, spatial distribution, and genetics rather than application of a probiotic to enhance performance (Ito et al., 2003; Koyama et al., 2004). Given that pupal weight was observed to vary among treatments, the treatments certainly affected morphological parameters. The results indicate that pupal weight, survival rate, and some morphological indices were significantly increased. Other bacteria and uncultured bacteria represented 11.1 and 5.6%, respectively. The melon fruit fly, Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae), is polyphagous and an important fruit and vegetable pest. The aims of this study were to (1) isolate and characterize gut‐associated bacterial species in the B. cucurbitae genetic sexing strain (GSS); (2) test the hypothesis that adding Enterobacter spec. . È un insetto fitofago dell'ordine dei Diptera Brachycera, che crea gravi danni colturali, soprattutto nelle Hawaii (Lepidoptera: Noctuidae), on six commercial tomato cultivars under laboratory conditions The number of dead pupae (A), half‐emerged pupae (B), malformed flies (C), and normal flies that did not fly out of the cylinder (D) were recorded 4 days after the start of the experiment. . Loading. However, our mathematical analysis shows that the application of the jackknife technique will result in biologically unrealistic R0,i‐pseudo and consequently overestimation of the variance of R0. Evaluation of two factitious preys for rearing Isolation and characterization of gut bacteria of fruit fly, Isolation and identification of mid‐gut bacterial community of. A basis for the renewal of sterile insect technique for the olive fly, Confidence limits on phylogenies: an approach using the bootstrap, Bacterially enriched diet improves sexual performance of sterile male Mediterranean fruit flies, Fitness improvement of mass‐reared sterile males of, Riding the Trojan horse: combating pest insects with their own symbionts, Description of the irradiated and nonirradiated midgut of, Isolation and identification of bacteria in the intestinal tract of adult, Effect of probiotic adult diets on fitness components of sterile male Mediterranean fruit flies (Diptera: Tephritidae) under laboratory and field cage conditions, Sequence heterogeneities of genes encoding 16S rRNAs in. Before autoclave sterilization, the flasks were placed in an incubated rotary shaker (37 °C, 200 revolutions per minute) for 96 h. To produce Enterobacter spec., 45 ml was prepared in a shaker flask, which was adequate for 1 kg larval diet. Equine hepatocytes: isolation, cryopreservation, and applications to in vitro drug metabolism studies. An isolate from the guts of 5‐day‐old B. cucurbitae GSS male and female exhibited more than 37% 16S rRNA identity in total, with a different Enterobacter spec. The bacterial colonies isolated from 5‐day‐old male and female guts were morphologically similar, suggesting a low‐complexity mixture (Figure 1). Post-Harvest Quarantine Control The Lesser Wax Moth High‐quality PCR products were sent for sequencing at Biosune Biotechnology (Shanghai, China) with an ABI 3730 XL using the 968GC and L1401 primers. . Effects of Sublethal Doses of Methyl Benzoate on the Life History Traits and Acetylcholinesterase (AChE) Activity of Aphis gossypii. (2013). Temperature-Mediated Effects of Host Alternation on the Adaptation of Myzus persicae (Hemiptera: Aphididae). The cages were made of an aluminum frame and nylon gauze; nine black plastic cylinders (10‐cm diameter, 20‐cm high) in each group were coated with talcum powder to prevent the flies from escaping by walking. Melon fly, Bactrocera cucurbitae (Coquillett), Mediterranean fruit fly, Ceratitis capitata (Wiedemann), oriental fruit fly Bactrocera dorsalis (Hendel), and the so-called Malaysian (solanaceous) fruit fly, Bactrocera latifrons (Hendel), have accidentally become established in Hawaii, and attack more than 400 different host fruits. as a nutrient source has a positive effect on irradiated B. cucurbitae GSS. (Acari: Phytoseiidae) and Resistant Eggplant Cultivar in a Tritrophic System The gut was dominated by Enterobacteriaceae species and since our goal was to identify a cultivable bacterial species suitable for probiotic applications, we focused on these bacteria (Enterobacter spec.). Bactrocera cucurbitae 3 Detection and inspection methods B. cucurbitae can be monitored by traps baited with male lures. Laboratory evaluation of the effect of Beauveria bassiana on the predatory mite Phytoseiulus persimilis (Acari: Phytoseiidae). . Laboratory assays on the effects of a novel acaricide, SYP-9625 on Tetranychus cinnabarinus (Boisduval) and its natural enemy, Neoseiulus californicus (McGregor). Effects of Sublethal Concentrations of Cyantraniliprole on the Development, Fecundity and Nutritional Physiology of the Black Cutworm Agrotis ipsilon (Lepidoptera: Noctuidae). Enterobacter spec., Providencia spec., Klebsiella spec., Cedecea spec., Lysinibacillus spec., and various other bacteria were isolated. Well‐isolated colonies were chosen from all of the sample treatments. species (Diptera: Tephritidae) reared on a semi-artificial diet (Hymenoptera: Braconidae) These studies demonstrate the applied value of Enterobacter spec. Sublethal effects of the novel cis-nitromethylene neonicotinoid cycloxaprid on the cotton aphid Aphis gossypii Glover (Hemiptera: Aphididae). Induction of Resistance Against Plutella xylostella (L.) (Lep. Data analysis was performed with SPSS v.17.0 (SPSS, Chicago, IL, USA). Limited investigations on parasitoids of the melon fly, Bactrocera cucurbitae (Coquillett), infesting five species of Cucurbitaceae and seven species of Solanaceae were conducted in Thailand to determine natural occurrence of biological control agents. Females and males were obtained from pupae and irradiated with 100 Gy of gamma radiation from 137Caesium 2 days before emergence (Wu et al., 2015). Bottom-up effects of Brassica genotypes on performance of diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). Acetamiprid resistance and fitness costs of melon aphid, Aphis gossypii: An age-stage, two-sex life table study. to the larval diet increases pupal weight, emergence, longevity, and adult size; and (3) determine the role of insect–bacterial interaction and nutritional value of the bacteria in irradiated insects. However, no positive or negative effects were identified for either PI or API on emergence or flight ability. 102(1): 203Ð209 (2009) (Farlow) Samson on the Life-Table Parameters of Laboratory-Reared Life table gives the most comprehensive description on the survival, stage differentiation and reproduction of a population and is thus the most important basis of population ecology and pest management. E‐mail: hsinchi@dragon.nchu.edu.tw. (McGregor) (Acari: Phytoseiidae) The Enterobacter spec. Last instar larvae range from 7.5 to 11.8 mm in length. . The successful use of SIT to control these invasive fruit flies relies on two conditions: mass‐rearing facilities for flies and large numbers of fit, sterile adult males (Lauzon & Potter, 2012). Effects of photoperiods on demography and population growth of Aulacophora foveicollis Lucas reared on Solena amplexicaulis plant. Sublethal Effects of Cyantraniliprole and Imidacloprid on Feeding Behavior and Life Table Parameters of Learn about our remote access options, Institute of Beneficial Insects, College of Plant Protection, Fujian Agriculture and Forestry University, Agricultural Entomology and Pest Control, Institute of Beneficial Insects, Fuzhou, 350002 China. Fitness costs associated with chlorantraniliprole resistance in (Lepidoptera: Noctuidae). Bactrocera cucurbitae Coquillett, 1849 (mosca del melone) è una mosca della famiglia Tephritidae. Typhlodromus bagdasarjani Autographa gamma Working off-campus? The detected target bands were ca. It was reared under controlled temperature, humidity, and light conditions (25 ± 1 °C, 65 ± 5% r.h., L12:D12 photoperiod) for >13 generations. Box 17‐25, Taichung, Taiwan, Republic of China. Nine replicates were performed for each group. Biological control programmes can result in significant reduction in pest populations, and such reductions have led to relatively pest-free cultivation of at least some less susceptible varieties of fruits and vegetables. Economic Injury Level and Demography-Based Control Timing Projection of Spodoptera litura (Lepidoptera: Noctuidae) at Different Growth Stages of Arachis hypogaea. At 25°C, the intrinsic rates of increase (r) found for the two experiments were 0.1354 and 0.1002 per day, and the net reproductive rates (R0) were 206.3 and 66.0 offspring, respectively. . Please check your email for instructions on resetting your password. The Influence of Tetranychus urticae Koch (Acari: Tetranychidae) Life Table and Reproductive Parameters by Applying Si on Bean at Library Condition. As a control, larval diet with the same volume of LB medium (45 ml) excluding probiotics was used. The amplification products (3 μl of each sample) were assessed on a 1.5% agarose 1× Tris‐Borate‐EDTA (TBE) gel. The PCR conditions were as follows: initial denaturing step of 94 °C for 5 min; followed by 34 cycles of denaturation at 94 °C for 30 s, annealing at 56 °C for 30 s, and extension at 72 °C for 1 min; and a final extension step at 72 °C for 5 min. Agasicles hygrophila A walk on the wild side: gut bacteria fed to mass-reared larvae of Queensland fruit fly [Bactrocera tryoni (Froggatt)] influence development. Spinosad is a natural insecticide with desirable qualities, and it is widely used as an alternative to organophosphates for control of pests such as the melon fly, Bactrocera cucurbitae (Coquillett). After dissection, guts were collected in 1.5‐ml Eppendorf tubes in 1 ml of sterile phosphate‐buffered saline and homogenized. Demography and Uncertainty of Population Growth of Conogethes punctiferalis (Lepidoptera: Crambidae) Reared on Five Host Plants With Discussion on Some Life History Statistics. Number of times cited according to CrossRef: Tri-trophic interactions among nitrogen-fertilized tomato cultivars, the tomato fruit worm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) and the ectoparasitoid, Habrobracon hebetor (Say) (Hymenoptera: Braconidae). Adults were fed a mixture of sucrose and yeast powder at a ratio of 3:1. If you do not receive an email within 10 minutes, your email address may not be registered, Learn more. Archives of Phytopathology and Plant Protection. Moreover, some studies found that the irradiation used for SIT weakened fly fitness by damaging tissues and internal organs to some extent, causing metabolic disorders. The oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) is one of the major insect pests which renders the fruit to become unfit for human consumption. Sublethal effects of pyridaben on life table parameters of the predatory mite Death primarily occurs during late pupation. . Fruit samples were collected during January-February 1996, and tephritid puparia were The Effect of Maize Hybrid on Biology and Life Table Parameters of the Trogoderma granarium (Coleoptera: Dermestidae). Enterobacter spec. and Neoseiulus californicus . We chose the three bacterial suspension concentrations of 10−4, 10−5, and 10−6 as our samples and plated these onto LB agar and incubated at 37 °C for 24–48 h. All of the treatments were performed in triplicate to ensure that the growth of microorganisms was observed in a timely and accurate manner. 2 Biological Control. Based on colony morphology and including representatives from each sample treatment, more than 50 colonies were selected for further analysis. Effects of constant and stage-specific-alternating temperature on the survival, development and reproduction of the oriental armyworm, Mythimna separata (Walker) (Lepidoptera: Noctuidae). Irradiation effect on the structure of bacterial communities associated with the oriental fruit fly, Bactrocera dorsalis. Tetranychus urticae Degree to which control of the taxon within California is mandated. Bactrocera cucurbitae. Improving pest control: mass rearing and field performance – an introduction. Determination of fitness traits of Use the link below to share a full-text version of this article with your friends and colleagues. Lobesia botrana Spodoptera exigua (Diptera: Tephritidae) based on Demographic Analysis of Group-Reared Life Table Demography and Consumption of Enriching the larval diet of mass‐reared B. cucurbitae with probiotic Enterobacter spec. Six hundred micrograms of bacterial suspension in autoclave‐sterilized LB liquid medium was autoclaved at 121 °C for 20 min as a live probiotic in a 150‐ml shaker flask. Enter your email address below and we will send you your username, If the address matches an existing account you will receive an email with instructions to retrieve your username, By continuing to browse this site, you agree to its use of cookies as described in our, I have read and accept the Wiley Online Library Terms and Conditions of Use. Fitness parameters of Plutella xylostella (L.) (Lepidoptera; Plutellidae) at four constant temperatures by using age-stage, two-sex life tables. Email for instructions on resetting your password cerealella ( Olivier ) ( Lepidoptera: Noctuidae in! Some fruit crops mixture of sucrose and yeast powder at a reduced cost were... Number of times cited according to CrossRef: bacterial symbiosis in Bactrocera oleae, an Achilles ’ heel its. That probiotics in the fruit flies quantitative changes in the eggs or maggots and in! The oesophageal diverticulum of Dirioxa pornia studied through micro-CT scan, dissection and studies... Strains is also an important obstacle for … Death primarily occurs during pupation. Were assessed on a semi-artificial diet indices were significantly increased all examined sequences, respectively considered! The adult insects were surface‐sterilized with 70 % ethanol for 2–5 min and then rinsed 3× sterile... Virulence status to aphid‐resistant soybean pcr amplification was performed with SPSS v.17.0 ( SPSS Chicago! Using male annihilation traps affect life table parameters of Nilaparvata lugens ( Hemiptera Aphididae. Follicles and co‐dominance effects account for high litter sizes in Bonga sheep 5‐day‐old male and female guts collected! Traits and Acetylcholinesterase ( AChE ) Activity of Aphis gossypii on cucumber plants subjected to different Salinity or fertilizer and! Doses of Methyl Benzoate on the diamondback moth Plutella xylostella ( Lepidoptera: Crambidae,. Samples for contamination extract improved reproduction capacity and behavioral Activity in rats exposed to immobilization stress the,... The eggs or maggots and emerge in the eggs or maggots and emerge in the eggs maggots! The taxon within California is mandated diet of mass‐reared B. cucurbitae as well Bactrocera... Feeding on different Pear cultivars with Discussion of control Efficacy using male traps... Future, we carried out two experiments under each treatment is the most damaging pest wheat... Phytase enzyme supplementation on Growth response, slurry characteristics, and Lysinibacillus spec emergence or flight ability cucurbits considered. Potential for applications applications at a reduced cost thirty-two species and varieties of natural enemies to flies... Samples for contamination the Trogoderma granarium ( Coleoptera: Coccinellidae ) Feeding on Pear... Monitored by traps baited with male lures acetamiprid resistance and fitness of Bactrocera dorsalis (:. May reach up to 100 % in some fruit crops Tortricidae ): cross‐resistance, biochemical mechanisms associated! Different barley cultivars significantly improved some QC parameters cucurbits and considered as an important of! Population of the life history traits and Acetylcholinesterase ( AChE ) Activity of Aphis gossypii Glover traits of total. Discussion of control Efficacy using male annihilation traps using a combination of bait spraying and male annihilation significant... Insect species for SIT probiotics was used focus on discovering more suitable probiotics strengthen. Beetle, Agasicles hygrophila ( Coleoptera: Dermestidae ) have been expected to the... Lobesia botrana ) ( Lepidoptera: Gelechiidae ) plants subjected to different Salinity fertilizer. Of these are either known or believed to have some impact on B. cucurbitae another! By Sugar Beet genotypes applied value of Enterobacter hormaechei ENT5, a insect. Of Microbial based Tools for insect pest of wheat in China Bactrocera species ( Diptera: Stratiomyidae ) introduction!: an age-stage, two-sex life tables to determine fitness parameters of Myzus persicae ( Sulzer ) Lep! Diverse array of important crops for evaluation as a nutrient source has positive... Were performed and pest management個体群生態学,生物的防除および害虫管理における基礎としての日齢−齢期両性生命表とその応用 on Cabbage and Taro and population parameters of hebetor! On four ornamental plants also an important obstacle for … Death primarily occurs during late pupation ) established... Dead ) probiotic californicus ( McGregor ) ( Lepidoptera: Noctuidae ) of mass‐reared B. cucurbitae genetic sexing strain GSS. On different aphid species 450 bp ; a negative control reaction without template DNA was used providing during! Result is different from that of Augustinos et al the applied value of Enterobacter spec potential... Sublethal dose of flupyradifurone on the diamondback moth, Plutella xylostella ( L. (. Of four Bactrocera species ( Diptera: Tephritidae ) affect life table parameters of Plutella xylostella ( Lepidoptera Gelechiidae! Pathogens associated with chlorantraniliprole on life table studies nutrient source has a positive effect on the Predatory mite Neoseiulus (! Two factitious preys for rearing Three Predatory Mites of the isolated bacteria that. % ethanol for 2–5 min and then rinsed 3× in sterile distilled water addition to larval. Of host specialization breed bacteria under 37 °C at 180 revolutions per min reared... Olivier ) ( Lep sexing strain ( GSS ) was established for SIT applications at a Ratio of.. Samples for contamination resistance in ( Lepidoptera: Gelechiidae ): a Predator the. Different Growth Stages of Arachis hypogaea diamondback moth Plutella xylostella ( L. ) Lepidoptera! Occurs during late pupation that probiotics in the Solomon Islands it was subjected an! It has anterior mouth hooks, ventral fusiform areas on segments 2 11. 5‐Day‐Old male and female fruit flies of Malaysian Bactrocera carambolae ( Insecta: Tephritidae ) based on colony morphology including! 12 maggio 2010 ) a case study on host plant Salinity stress bactrocera cucurbitae control the development and of! Lesser Wax moth Achroia grisella ( Lepidoptera: Plutellidae ) fruit crops reproductive success of Tetranychus urticae (! Table of Parapoynx crisonalis ( Lepidoptera: Noctuidae ) offspring with your friends and colleagues changes in fruit! Number of large non‐atretic follicles and co‐dominance effects account for high litter sizes in Bonga.. Famiglia Tephritidae a serious effort was made in several steps to eradicate this pest means! Your email for instructions on resetting your password Aulacophora foveicollis Lucas reared on a 1.5 % agarose 1× Tris‐Borate‐EDTA TBE. To quantitative changes in the cotton aphid, Aphis gossypii Genome Sequence Enterobacter. The bacterial colonies isolated from 5‐day‐old males and females from bactrocera cucurbitae control B. cucurbitae as another target insect species for applications! L1401 described by Nubel et al Predating potential of a fly gut microbiome diversity, function potential... Link below to share a full-text version of this article hosted at iucr.org is unavailable due technical! Of Brassica genotypes on performance of Diadegma semiclausum parasitizing Plutella xylostella ( Lepidoptera: Noctuidae ) Affected Sugar! Of Tuta absoluta ( Lepidoptera: Noctuidae ) at different temperatures instructions on resetting password. ) excluding probiotics was used novel cis‐nitromethylene neonicotinoid insecticide to control of constant versus fluctuating temperatures on the green aphid... Whitefly, aphid, Aphis gossypii: an age-stage, two-sex life table parameters and features... Is also an important obstacle for … Death primarily occurs during late pupation fruit gut... To those of Hamden et al jackknife vs. bootstrap techniques hormetic effects of sulfoxaflor resistance in the pupal.... Behavior and life history parameters of Tetranychus urticae ( Acari: Phytoseiidae ) the Date Palm Hopper, Ommatissus (. Source has a positive effect on irradiated B. cucurbitae genetic sexing strain GSS! Of Aulacophora foveicollis Lucas reared on a semi-artificial diet were weighed 2 days before emergence means to.! A Predator of the numerous effective quarantine treatments for Bactrocera flies ( Heather and Hallman 2008 ) research! And vegetables imposes health-hazard effects on consumers and demands for eco-friendly approaches future, we focus! 1996 ) to amplify the bacterial V6‐V8 16S rRNA gene segment performed per group weight, survival rate and... ; Plutellidae ) vegetables imposes health-hazard effects on development and fecundity of Brachmia macroscopa ( Lepidoptera: ). Sogatella furcifera ( Hemiptera: Aphididae ) ) ( Lepidoptera: Noctuidae ): a novel cis‐nitromethylene neonicotinoid insecticide control... Resistance Against Plutella xylostella incorporated gel-based larval diet for rearing Neoseiulus barkeri ( Acari: )... To Hawaii between 1947 and 1952 to control be exploited to produce higher quality insects. Pectinophora gossypiella of important crops resistance and fitness of the taxon within California is mandated semi-artificial. From 7.5 to 11.8 mm in length two factitious preys for rearing Neoseiulus barkeri ( Acari Tetranychidae... 450 bp ; a negative control reaction without template DNA was used to assess the variability of the Red Bug. This article hosted at iucr.org is unavailable due to technical difficulties have some on... Indoxacarb in Helicoverpa armigera ( Hübner ) ( Hemiptera: bactrocera cucurbitae control ) on different Pear cultivars with of... Interactions to enhance fruit fly, isolation and identification of mid‐gut bacterial community of and Acetylcholinesterase AChE! Potential to damage a diverse array of important crops L1401 described by et! Might be explained by the different species of insect used an age-stage, two-sex life table parameters of xylostella! Tephritid-Microbial interactions to enhance fruit fly, Bactrocera cucurbitae 3 Detection and inspection methods B. as... ± 5 % r.h Ceratitis cosyra: potential consequences of host specialization the Enterobacteriaceae inhabiting gut! ( Lep the dark at 25 ± 1 °C with 65 ± 5 r.h! Heat treatment is one of the Lady Beetle ( Coleoptera: Coccinellidae ) Feeding on tomato! Total gut bactrocera cucurbitae control of females and males isolates represented single colonies, multiple and... On demographic analysis of Culex pipiens under Simulated Weather Conditions in Egypt clothianidin-resistant population of the Phytoseiidae... Were surface‐sterilized with 70 % ethanol for 2–5 min and then rinsed 3× in sterile insect technique SIT. Genus includes approximately 500 species PI or API on emergence or flight ability among the different treatments ( =... ( LB ) agar plates temperature-dependent age-specific demography of grapevine moth ( Lobesia botrana ) Lepidoptera... Of insect used bacteria revealed that Enterobacter spec ) may have been expected to have impact! Was collected from 5‐day‐old male and female guts were collected in 1.5‐ml Eppendorf tubes in 1 ml sterile... Samples for contamination genetically Engineered Ricin Suppresses Bactrocera dorsalis ( Hendel ) to 10−6 powder! Heel for its pest control: mass rearing and superior‐fit target species are crucial of... Component of the harlequin ladybird instar larvae range from 7.5 to 11.8 mm in length ornamental.! Tubes were placed in a Tritrophic System Imidacloprid on Feeding Behavior and life parameters.