(2011), Kamala Jayanthi PD, Vivek K, Ravindra MA, Ravindra KV, Bakthavatsalam N, Verghese A, et al. Salicylic acid, hydrogen peroxide, catechol, guaiacol, polyvinylpyrrolidone, tris buffer, sodium phosphate, and di-sodium phosphate were purchased from Himedia, Bangalore, India. Identification of neryl formate as an airborne aggregation pheromone for the American house dust mite, Gas chromatographic characterization of organic substances in the retention index system, A colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, Chun OK, Kim DO, Moon HY, Kang HG, Lee CY (2003), Contribution of individual polyphenolics to total antioxidant capacity of plums, Lowry H, Rosebrough NJ, Farr AL, Randall RJ (1951), Protein measurement with the Folin phenol reagent, Effect of pre-and post-harvest application of salicylic acid or methyl jasmonate on inducing disease resistance of sweet cherry fruit in storage, Evolution of oviposition behavior and host preference in Lepidoptera, Host-plant selection by phytophagous insects, Pickett JA, Wadhams LJ, Woodcock CM (1998), Insect supersense: mate and host location as model systems for exploiting olfactory mechanisms, Perception of plant volatile blends by herbivorous insects-finding the right mix. stream Similarly, the salicylic acid treatment resulted in increased endogenous H2O2 level that involved in resistance against H. armigera [73]. Kamala Jayanthi Pagadala Damodaram, Ravindra Mahadappa Aurade, [...], and Abraham Verghese. In the present study, we also observed changes in antioxidative enzymes such as catalase, PO and PPO. Furthermore, host recognition depends on blends or ratios of volatiles emitted rather than the presence or absence of individual compounds [54]. Post hoc analyses employing Bonferroni correction revealed that the numbers of oviposition punctures and eggs per puncture were significantly less in SA treated fruit irrespective of post SA treatment time intervals [1 day (oviposition punctures: t = 3.78, P<0.01; eggs per puncture: t = 4.67, P <0.001), 2 days (oviposition punctures: t = 3.67, P <0.01; eggs per puncture: t = 2.86, P <0.05), 3 days (oviposition punctures: t = 4.50, P <0.01; eggs per puncture: t = 4.20, P <0.01)] (Fig 2a and 2b). Toggle facets Limit your search Text Availability. Individual volatile compounds were identified by comparing the retention index that was calculated by using homologous series of n-alkanes (C5 to C32 procured from Sigma-Aldrich) as standard [44] and comparing the MS spectra with spectral libraries (Wiley and NIST-2007). 2 <>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/Annots[ 7 0 R 24 0 R] /MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S>> We analyzed the activity of antioxidative enzymes viz., catalase, peroxidase, polyphenoloxidase in both SA treated and untreated fruit respectively (n = 3). The number of eggs laid per fruit was also significantly (0.5 mM: t = 5.15, df = 4, P = 0.01; 1.0 mM: t = 9.57, df = 4, P = 0.001; 2.0 mM: t = 5.49, df = 4, P = 0.01; 5 mM: t = 4.78, df = 4, P = 0.01) lower in SA treated fruit compared to controls (see *Significantly different from SA treated and control arms; n.s. Thus, in the present study, SA application not only reduced the attraction of host fruit to fruit fly, B. dorsalis but also affected the larval development and subsequent adult emergence indicating SA treatment enhances mango tolerance to the Oriental fruit fly. The authors duly acknowledge the constructive comments of the anonymous reviewers and editor which immeasurably improved the manuscript. Further, GA3 application was found to offer viable means for reducing grapefruit susceptibility to A. suspensa in the field by reducing fruit attractiveness, ovipositional acceptability and physiological suitability for larval development by delaying peel senescence (37–39). The observed increase in total phenol/flavonoid compounds would have been responsible for poor larval development and reduced adult emergence of B. dorsalis [66]. detected from mango fruits. The damage from this pest is caused by the larvae that bore inside the fruits. Keywords Mango, Mangifera spp, Mangifera casturi, Mangifera lalijiwa, Bactrocera dorsalis, mango relative. To identify a suitable oviposition site, insects mainly depend on host released volatile blends [2], particularly, during oviposition site selection [55]. Among the elicitors listed above, SA is well studied in non-woody plants for its role in regulating plant defense and in triggering ‘systemic acquired resistance’ (SAR) [14–19]. Contributed reagents/materials/analysis tools: TKR KSS AV. %PDF-1.5 Wrote the paper: KJPD RMA VK. This is the first time we show that the SA induced changes in mango fruit affects the attraction of female Oriental fruit fly, B. dorsalis. The concentrated samples were stored in screw-capped bottles at 4°C until further use. Application of SA on the fruit in field and exposing those fruit to fruit flies under laboratory conditions also exhibited similar trend, indicating SA treated fruit were less acceptable to B. dorsalis. The ePub format is best viewed in the iBooks reader. Headspace volatiles from SA treated and untreated mango fruit of green mature stage (cv. However, use of host plant defense pathways that can be switched on or primed by exogenous application of phytohormones is not explored in mango. Elakki) for six generations, with one generation cycle of one month and maintained at ambient conditions (27 ± 1°C, 75 ± 2% RH and 14L: 10D h photoperiod) in the Fruit Entomology Laboratory, Division of Entomology and Nematology, Indian Institute of Horticultural Research (IIHR), Bangalore, India [40]. Conceived and designed the experiments: KJPD RMA. (a) punctures (b) Eggs per puncture. The fruit were allowed to develop on the trees. Citation in PubAg 449; Full Text 83; Journal. Salicylic acid (SA) is an important phytohormone that mediates the phenylpropanoid pathway and is known to promote the release of many intermediary secondary metabolites and anti-nutritive compounds in plants [14, 20]. Totapuri, a susceptible variety to B. dorsalis) [41] fruit were collected from the experimental orchard of the Indian Institute of Horticultural Research, Bangalore. Innate recognition of pheromone and food odors in moths: A common mechanism in the antennal lobe? Further studies on effect of SA on larval development and adult emergence of B. dorsalis revealed significant reduction in pupae formation and adult emergence when larvae were reared on SA treated fruit (Fig 3b). Total volatile production was estimated by the sum of all GC-FID peak areas in the chromatogram and individual compounds were quantified as relative percent area. It is native to large parts of tropical Asia, has become established over much of sub-Saharan Africa, and is often intercepted in the United States, sometimes triggering eradication programs. Exogenous treatment of SA to host plants, induced defense and modified the behavior of cotton bollworm, Helicoverpa armigera (Hübner) and the mite, Tetranychus urticae Koch [25,26]. A simple and cost-effective mass rearing technique for the tephritid fruit fly. Volatile chemical cues from the host plant play a major role in the orientation of gravid females to their hosts from a distance. [42]. Insects use an array of volatile compounds as cues to locate food, mates and oviposition sites [1–4]. C'est une mouche des climats tropicaux chauds et humides qui pond ses œufs dans les fruits charnus de plus de 400 espèces de plantes sauvages ou cultivées, tant d'arbres fruitiers, notamment les mangu… (2009), Signal signature of above ground induced resistance upon below ground herbivory in maize, Zhao LY, Chen JL, Cheng DF, Sun JR, Liu Y, Tian Z (2009), Biochemical and molecular characterizations of, Bruinsma M, Van Dam NM, Van Loon JJA, Dicke M (2007), Induced resistance in groundnut by jasmonic acid and salicylic acid through alteration of trichome density and oviposition by, Fine-tuning plant defence signalling: Salicylate versus jasmonate, Postharvest salicylic acid treatment reduces chilling injury of ‘Taify’ cactus pear fruit during cold storage, Ryals JA, Neuenschwander UH, Willits MG, Molina A, Steiner HY, et al. Were subjected to paired t test lignin concentration in plants [ 21, 22 ] mechanism... 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